Investigation of Polystyrene, Polyisoprene, and Poly(2-vinylpyridine) using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
نویسندگان
چکیده
MALDI-TOF-MS is a soft ionization technique that has the potential to determine the molecular weight for various compounds, particularly for large biomolecules.1-3 Recently, considerable interest has been focused on the analysis of synthetic polymers by MALDI-MS.4 Compared with the traditional methods of polymer analysis, MALDI-MS can provide more abundant information on molecular weight, repeat unit, end group, additives, impurities, and side products for pure polymers, and even on complex copolymers with recently improved resolution and mass accuracy.5-7 Although this new method is fast, popular, and instrumentally very simple, MALDI results obtained by different groups for polymer analysis have revealed different conclusions.8,9 This comes from considerable variations in sample preparation and instrumental limits.10,11 Recently, Schriemer groups reported the detection of very high molecular weight narrow polydisperse polystyrene by careful formulation of the matrix/analyte preparation and by using a sensitive MALDI detection system.12 This paper discusses the matrix effect, cationization agent effect, and solvent effect with the different molecular weight polystyrenes used as GPC standards. UV absorption and fluorescence properties of matrices are studied to get the fundamental understanding about the MALDI mechanism of ion formation using an UV laser. This work also demonstrates that MALDI can be a useful tool for polyisoprene and poly(2-vinylpyridine) molecular weight determination using proper matrix selection and sample preparation.
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